Résumé :
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In Chromatin Protocols, Peter Becker and a panel of eminent investigators offer a collection of state-of-the-art methods designed to analyze chromatin structure and function, and the relationship between them. These proven methods cover a wide range of topics, from powerful cell- free systems for the reconstitution of chromatin heterogeneity in vitro, to both classical and cutting-edge techniques for in vivo analysis of protein-DNA interactions. Each method includes detailed step-by-step instructions to ensure successful execution, along with helpful notes about how to avoid pitfalls. All authors are leading scientists, well-known for their methodological expertise in chromati n research. Chromatin Protocols provides a collection of basic and state-of-the-art techniques suitable for use in all laboratories carrying out chromatin research, as well as for novices entering the field. Essential to all future chromatin studies, its powerful proven methods are even now driving elucidation of the molecular mechanisms that control such vital cellular functions as transcription, replication, recombination, and DNA repair.
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